Proteomics as the final step in the functional metagenomics study of antimicrobial resistance

Review that might be of interest to some:

The majority of clinically applied antimicrobial agents are derived from natural products generated by soil microorganisms and therefore resistance is likely to be ubiquitous in such environments. This is supported by the fact that numerous clinically important resistance mechanisms are encoded within the genomes of such bacteria. Advances in genomic sequencing have enabled the in silico identification of putative resistance genes present in these microorganisms. However, it is not sufficient to rely on the identification of putative resistance genes, we must also determine if the resultant proteins confer a resistant phenotype. This will require an analysis pipeline that extends from the extraction of environmental DNA, to the identification and analysis of potential resistance genes and their resultant proteins and phenotypes. This review focuses on the application of functional metagenomics and proteomics to study antimicrobial resistance in diverse environments.

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Kin cell lysis is a danger signal that activates antibacterial pathways of Pseudomonas aeruginosa

New eLife paper from the Mougous lab with Brook Peterson as a middle author, on how Pseudomonas cells can sense death of their neighbors and changes their fitness in co-culture.

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Phage-mediated Dispersal of Biofilm and Distribution of Bacterial Virulence Genes Is Induced by Quorum Sensing

We’ll be going over the following paper in journal club this week with a discussion led by Megan:

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NYC Subway Microbiome Paper

A new paper in Cell explores the microbiome  (and human metagenomes, if that’s the appropriate term) of NYC’s subways:

Also, the NY Times coverage of this article is trending on facebook! Everybody <3s microbes!

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Growth and host interaction of mouse segmented filamentous bacteria in vitro

New article from Sansonetti’s group; they’ve found a way to culture SFBs in vitro in coculture with mouse cells and can now further study how SFBs influence the host immune system.

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Quorum sensing triggers the stochastic escape of individual cells from Pseudomonas putida biofilms

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Dermal adipocytes protect against invasive Staphylococcus aureus skin infection

Jessica brought this paper to my attention; a group at UCSD found that fat cells in the skin protect against Staph aureus infection by producing an antimicrobial peptide.  Nice story, and there’s an accompanying commentary article that is also a good read.  They suggest that while it isn’t known how the adipocytes recognize Staph or other bacteria and know to produce the AMP, it could be happening through toll-like receptor signaling, since fat cells have TLRs.  Didn’t know that!  Cool stuff.

Primary article:

Commentary article, “Killer Fat”:

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