Monthly Archives: August 2012

Second Article

By the way, Ezra here (forgot to say so in the previous post.).  The second article I found because I saw Professor Carlson speak at Yale several months ago and was very impressed.  In this paper, they synthesized cephalosporin analogs … Continue reading

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Two Articles

Hi JoLab!   I miss all of you, and I’ve read some interesting items recently: First, a hospital study, where genome sequencing was used to track a Klebsiella outbreak.  Despite the most rigorous precautions, the infection spread to multiple patients. … Continue reading

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Determination of Whether Quorum Quenching Is a Common Activity in Marine Bacteria by Analysis of Cultivable Bacteria and Metagenomic Sequences

I thought that some of you might be interested in this article. It’s interesting and there are a lot of seemingly good references and technical information. Abstract: The abundance of quorum quenching (QQ) activity was evaluated in cultivable bacteria obtained … Continue reading

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Microdroplet-enabled highly parallel co-cultivation of microbial communities

by Park et al. 2011 PLoS One



Microbial interactions in natural microbiota are, in many cases, crucial for the sustenance of the communities, but the precise nature of these interactions remain largely unknown because of the inherent complexity and difficulties in laboratory cultivation. Conventional pure culture-oriented cultivation does not account for these interactions mediated by small molecules, which severely limits its utility in cultivating and studying “unculturable” microorganisms from synergistic communities. In this study, we developed a simple microfluidic device for highly parallel co-cultivation of symbiotic microbial communities and demonstrated its effectiveness in discovering synergistic interactions among microbes. Using aqueous micro-droplets dispersed in a continuous oil phase, the device could readily encapsulate and co-cultivate subsets of a community. A large number of droplets, up to ~1,400 in a 10 mm×5 mm chamber, were generated with a frequency of 500 droplets/sec. A synthetic model system consisting of cross-feeding E. coli mutants was used to mimic compositions of symbionts and other microbes in natural microbial communities. Our device was able to detect a pair-wise symbiotic relationship when one partner accounted for as low as 1% of the total population or each symbiont was about 3% of the artificial community.


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